cell lines hmc3 human microglial cell line atcc crl 3304 bt0309 mouse brain tumor  (ATCC)

Journal: Glia

Article Title: Endoplasmic Reticulum Stress Amplifies Cytokine Responses in Astrocytes via a PERK / eIF2α / JAK1 Signaling Axis

doi: 10.1002/glia.70067

Figure Lengend Snippet: JAK1 and PERK are required to augment TNF‐α induced gene expression in human glioma cells. (A) 1321N1 cells stably expressing Cas9 were transfected with non‐targeting (NT) or JAK1 guide RNAs (gRNA) to establish non‐clonal cell lines. These cells were then treated with IFN‐γ (10 ng/mL) for 30 min followed by immunoblotting. (B) NT and JAK1 gRNA (#2) cells were treated with thaps (1 μM), TNF‐α (10 ng/mL), or both for 4 h followed by qPCR. (C) 1321N1 cells stably expressing Cas9 were transfected with NT or PERK gRNA to establish non‐clonal cell lines. These cells were then treated with thaps (1 μM) for the indicated times followed by immunoblotting. (D) NT and PERK gRNA cells were treated with thaps (1 μM), TNF‐α (10 ng/mL), or both for 4 h followed by qPCR. N = 4, data are means ± standard deviation.

Article Snippet: 1321N1 human astrocytoma cell line stably expressing Cas9 nuclease was purchased from GeneCopoeia.

Techniques: Gene Expression, Stable Transfection, Expressing, Transfection, Western Blot, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Anti-Proliferative Potential of Cynaroside and Orientin—In Silico (DYRK2) and In Vitro (U87 and Caco-2) Studies

doi: 10.3390/ijms242316555

Figure Lengend Snippet: Anti-proliferative potential of 7-Lut and 8-Lut tested on human tumor glial cell line U87, by MTS assay. ( A , B ) cell viability after 24 h of exposure to 7-Lut and 8-Lut. ( C , D ) cell viability after 48 h of exposure to 7-Lut and 8-Lut. ( n = 3). Notation (*) means results without statistical significance ( p > 0.05); (**) means results with statistical significance (0.05 < p < 0.01); (***) means results with high statistical significance ( p < 0.01); 7-Lut means luteolin-7- O -glucoside; 8-Lut means luteolin-8- C -glucoside; MTS means 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy- phenyl)-2 -(4-sulfophenyl)-2H-tetrazolium (MTS).

Article Snippet: The cell lines investigated were the human tumor brain cell line U87 MG(ATCC-HTB-14) and the human colorectal carcinoma Caco-2 cells (ATCC, HTB-37).

Techniques: MTS Assay

Journal: International Journal of Molecular Sciences

Article Title: Anti-Proliferative Potential of Cynaroside and Orientin—In Silico (DYRK2) and In Vitro (U87 and Caco-2) Studies

doi: 10.3390/ijms242316555

Figure Lengend Snippet: The viability response of U87 cells to 7-Lut and 8-Lut after 24 and 48 h of exposure.

Article Snippet: The cell lines investigated were the human tumor brain cell line U87 MG(ATCC-HTB-14) and the human colorectal carcinoma Caco-2 cells (ATCC, HTB-37).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of test reference compounds (caffeic acid, gentisic acid, ferulic acid and para-aminobenzoic acid/PABA) on human normal brain cell line NHA and human tumor brain cell line U87, in cytotoxicity MTS assay. ( A ) NHA cell line viability after 24 h of exposure to test reference compounds. ( B ) U87 cell line viability after 24 h of exposure to test reference compounds. Data were the mean of three replicates (n = 3). Note: * = results without statistical significance ( p > 0.05); ** = results with statistical significance ( p < 0.05).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of test reference compounds (caffeic acid, gentisic acid, ferulic acid and para-aminobenzoic acid/PABA) on human tumor brain cell line U87, in anti-proliferative MTS assay. ( A ) U87 cell line viability after 24 h of exposure to test reference compounds. ( B ) U87 cell line viability after 48 h of exposure to test reference compounds. Data were the mean of three replicates (n = 3). Note: * = results without statistical significance ( p > 0.05); ** = results with statistical significance ( p < 0.05).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of crude ethanolic extract from fresh Anemone nemorosa L. plant material (AN1) on human normal brain cell line NHA and human tumor brain cell line U87, in cytotoxicity MTS assay. ( A ) NHA cell line viability after 24 h of exposure to test vegetal extract AN1. ( B ) U87 cell line viability after 24 h of exposure to test vegetal extract AN1. All determinations were made in triplicate series, and the results are calculated as an average. Graphs A and B also present the percentage of cell viability by comparison to negative control series (CN). According to the statistical calculation, the results of the NHA and U87 cell lines in the cytotoxicity experiment were not statistically significant ( p > 0.05, n = 3).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of crude ethanolic extract from fresh Anemone nemorosa L. plant material (AN1) on human tumor brain cell line U87, in anti-proliferative MTS assay. ( A ) U87 cell line viability after 24 h of exposure to test vegetal extract AN1; ( B ) U87 cell line viability after 48 h of exposure to test vegetal extract AN1. All determinations were made in triplicate series, and the results are calculated as an average. The graphs present the percentage of the cell viability in series by comparison to positive control sample (CP) series. According to statistical calculation, the results on the U87 cells at 24 h and 48 h were not statistically significant ( p > 0.05).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of hot ethanolic extract from dried Anemone nemorosa L. plant material (AN2) on human normal brain cell line NHA and human tumor brain cell line U87, in cytotoxicity MTS assay. ( A ) NHA cell line viability after 24 h of exposure to test vegetal extract AN2. ( B ) U87 cell line viability after 24 h of exposure to test vegetal extract AN2. All determinations were made in triplicate series, and the results are calculated as an average. The graphs present the percentage of cell viability in each dilution point series by comparison to negative control point series (CN). By statistical calculation, the results on the NHA cell line were not statistically significant ( p > 0.05, n = 3), while the results on the U87 cell lines were statistically significant ( p < 0.05, n = 3).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of hot ethanolic extract from dried Anemone nemorosa L. plant material (AN2) on human tumor brain cell line U87, in anti-proliferative MTS assay. ( A ) U87 cell line viability after 24 h of exposure to test vegetal extract AN2. ( B ) U87 cell line viability after 48 h exposure to test vegetal extract AN2. All determinations were made in triplicate series, and the results are calculated as an average. The graphs present the percentage of cell viability in each dilution point series by comparison to negative control point series (CN). By statistical calculation, the results on the U87 after 24 h of exposure to AN2 are statistically significant ( p < 0.05, n = 3), while the results at 48 h were not statistically significant ( p > 0.05, n = 3).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Negative Control